Wednesday, February 29, 2012

What is PVB19 IgM ELISA Kit?

BACKGROUND: Infections with parvovirus B19 (PVB19) can cause significant morbidity in transplant recipients.
METHODS: To characterize the epidemiology and clinical spectrum of posttransplant PVB19 IgM ELISA Kit, we reviewed all cases at our institution during a 16-year period, summarized the data from 91 cases published in the medical literature, and performed longitudinal molecular surveillance for PVB19 DNAemia among 47 solid organ and hematopoietic stem cell transplant recipients.
RESULTS: The median time to onset of PVB19 disease was 7 weeks after transplantation. Anemia, leukopenia, and thrombocytopenia were present in 98.8%, 37.5%, and 21.0% of patients, respectively. Hepatitis, myocarditis, and pneumonitis were also reported in association with PVB19 disease. Allograft tissue loss or dysfunction was observed at the time of PVB19 disease in 10% of cases. At the onset of disease, PVB19 IgM ELISA Kit serological test results were negative in 29% of cases. Almost all patients (96%) with anti-PVB19 IgM had a positive PVB19 polymerase chain reaction assay result. Intravenous immunoglobulin was the most commonly used treatment modality. Three of 98 patients died of myocarditis and cardiogenic shock associated with PVB19 disease. Molecular surveillance throughout the first year after transplantation did not reveal PVB19 DNAemia in 47 anemic solid organ and hematopoietic stem cell transplant patients.
PVB19 is a rare but clinically significant infection that manifests as refractory anemia during the posttransplantation period. The use of polymerase chain reaction for diagnosis is particularly helpful in immunosuppressed transplant patients who may fail to mount antibodies against PVB19 during active infection.
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Tuesday, February 28, 2012

What is sPLA2 ELISA Kit?

Intended use of sPLA2 ELISA Kit
This immunoassay kit allows for the specific measurement of human secreted phospholipase A2, sPLA2 concentrations in cell culture supernates, serum and plasma.
Introduction
Phospholipase A2 (PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid. The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell.
Test principle
sPLA2 ELISA Kit employs the quantitative sandwich enzyme immunoassay technique. A antibody specific for sPLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sPLA2 present is bound by the immobilized antibody. An enzyme-linked antibody specific for sPLA2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sPLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Description
This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin and mucous membranes. In case of contact, rinse affected area with plenty of water. Observe all federal, state and local regulations for disposal. 
All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.
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Monday, February 27, 2012

Description of Anti--HCMV PP65/HHV5 PP65(Human Cytomegalovirus PP65)

Anti--HCMV PP65/HHV5 PP65(Human Cytomegalovirus PP65)
Synonym: CMV, CMV glycoprotein B, CMV glycoprotein GP55, Cytomegalovirus glycoprotein GP55, gB, Glycoprotein B, Glycoprotein GP55, HCMV, HCMV glycoprotein B, HCMV glycoprotein GP55, HHV 5, HHV 5 glycoprotein B, HHV 5 glycoprotein GP55, HHV5 glycoprotein B, HHV5 glycoprotein GP55, Human herpesvirus 5 glycoprotein B, Human herpesvirus 5 glycoprotein GP55, UL55.
Description of Anti--HCMV PP65/HHV5 PP65(Human Cytomegalovirus PP65)
Cytomegalovirus is a herpes viral genus of the Herpesviruses group: in humans it is commonly known as HCMV or Human Herpesvirus 5 (HHV-5).CMV belongs to the Betaherpesvirinae subfamily of Herpesviridae, which also includes Roseolovirus.
 HCMV is also the virus most frequently transmitted to a developing fetus. HCMV infection is more widespread in developing countries and in communities with lower socioeconomic status and represents the most significant viral cause of birth defects in industrialized countries. CMV "seems to have a large impact on immune parameters in later life and may contribute to increased morbidity and eventual mortality.
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Thursday, February 23, 2012

Specifications of Follistatin ELISA Kit

Follistatin ELISA Kit
Tests: 1 x 96 well plate
Sample type
Cell culture supernatant, Urine, Serum, Plasma
Assay type: Sandwich
Sensitivity: < 0.5 ng/ml
Range: 0.512 ng/ml - 125 ng/ml
Recovery: 90%
Synonyms: CG12955, CG12956, Fol1, dFS, dFol1, Dmel\CG33466, CG33466, FS, xfs, MGC79635, FST, fst, LOC100033825, Fst, AL033346, FOL1, Fst-288, RATFOL1
Follistatin ELISA Kit(Enzyme-Linked Immunosorbent Assay) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Follistatin in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Follistatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Follistatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Follistatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Follistatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage    
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C . Avoid repeated freeze-thaw cycles.
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Wednesday, February 22, 2012

Where to buy Anti-SAP-1/FRG4(Synapse associated protein- I)?

Description:
SAP-1 is a member of the protein tyrosine phosphatase (PTP) family. The family comprises at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases.
SAP1 is a negative regulator of integrin-mediated signalling and has been implicated in carcinogenesis in many cancer types including gastro-intestinal and heptocellular cancers.
SAP-1 is a transmembrane protein and its gene is expressed primarily in brain and liver, and at a lower level in heart and stomach. It is also expressed in several cancer cell lines, but not in the corresponding normal tissues.
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Tuesday, February 21, 2012

Descriptions of BDNF ELISA Kit

Descriptions of BDNF ELISA Kit
Tests: 1 x 96 well plate
Sample type
Cell culture supernatant, Urine, Serum, Plasma
Assay type: Sandwich
Sensitivity: < 80 pg/ml
BDNF ELISA Kit is found in neurons of the central nervous system. It is expressed predominantly in hippocampus, cortex, and synapses of the basal forebrain. BDNF selectively supports the survival of primary sensory neurons and retinal ganglia. It supports survival and differentiation of certain cholinergic neurons and also some dopaminergic neurons in vitro. The factor inhibits the normal cell death of embryonic chick motor neurons.
The ab99978 Human BDNF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BDNF in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human BDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and BDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman BDNF antibody is added.
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Monday, February 20, 2012

Are you buying Troponin-T ELISA kit?


Troponin is a complex of three regulatory proteins that is integral to muscle contraction in skeletal and cardiac muscle, but not smooth muscle. Troponin is attached to the protein tropomyosin and lies within the groove between actin filaments in muscle tissue. Troponin is a component of thin filaments (along with actin and tropomyosin), and is the protein to which calcium binds to accomplish this regulation. Troponin has three subunits, Troponin C, Troponin I, and Troponin T. Individual subunits serve different functions. Troponin C binds to calcium ions to produce a conformational change in TnI. Troponin T binds to tropomyosin, interlocking them to form a troponin-tropomyosin complex. And in a less complicated light, Troponin T modulates contraction of striated muscle. Troponin I binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place.
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Troponin T, Tn-T concentrations in serum, plasma and other biological fluids.
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