Troponin is a complex of three regulatory proteins that is integral to muscle contraction in skeletal and cardiac muscle, but not smooth muscle. Troponin is attached to the protein tropomyosin and lies within the groove between actin filaments in muscle tissue. Troponin is a component of thin filaments (along with actin and tropomyosin), and is the protein to which calcium binds to accomplish this regulation. Troponin has three subunits, Troponin C, Troponin I, and Troponin T. Individual subunits serve different functions. Troponin C binds to calcium ions to produce a conformational change in TnI. Troponin T binds to tropomyosin, interlocking them to form a troponin-tropomyosin complex. And in a less complicated light, Troponin T modulates contraction of striated muscle. Troponin I binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place.
Troponin-T ELISA kit is a solid phase-phase sandwich enzyme linked immuno sorbent assay (ELISA). Samples, including standards of known target protein concentrations and unknowns are pipetted into these wells. During the first incubation, the target protein antigen and a biotinylated monoclonal antibody specific for target protein are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove the entire unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of target protein present in the samples.
Characteristics
Troponin-T ELISA kit allows for the in vitro quantitative determination of mouse Tn-T concentrations in cell culture supernates, serum, plasma and other biological fluids.
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