What is CD11b ELISA Kit?

Specificity    
CD11b ELISA Kit recognizes recombinant and natural rat Integrin alphaM. No significant cross-reactivity or interference was observed.
Principle    
The microtiter bowl provided in this kit has been pre-coated with an antibiotic specific to Integrin alphaM . Standards or samples are again added to the adapted microtiter bowl wells with a biotin-conjugated antibiotic alertness specific for Integrin alphaM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to anniversary microplate able-bodied and incubated. Again a TMB (3,3',5,5' tetramethyl-benzidine) substrate band-aid is added to anniversary well. Only those wells that accommodate Integrin alphaM , biotin-conjugated antibiotic and enzyme-conjugated Avidin will display a change in color. The enzyme-substrate acknowledgment is concluded by the accession of a sulphuric acerbic band-aid and the blush change is abstinent spectrophotometrically at a amicableness of 450 nm ± 2 nm. The absorption of Integrin alphaM in the samples is again bent by comparing the O.D. of the samples to the accepted curve.
Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, aswell accepted as macrophage-1 antigen (Mac-1) or accompaniment receptor 3 (CR3). ITGAM is aswell accepted as CR3A, and array of adverse atom 11B (CD11B). The additional alternation of αMβ2 is the accepted integrin β2 subunit accepted as CD18, and integrin αMβ2 appropriately belongs to the β2 subfamily (or leukocyte) integrins.
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