Pancreatic amylase is a digestive enzyme secreted by the pancreas. Pancreatic amylase consists of a single polypeptide chain with a molecular weight of 54 kDa. Pancreatic amylase occurs in serum at a concentration of about 60?g/L, from a total amylase denseness fashionable serum of approximately foresighted a hundred?constant of gravitation/L. Samples, including standards of known target protein concentrations and unknowns are pipetted into these Herbert George Wells. During the commencement incubation, the target protein antigen and a biotinylated monoclonal antibody specific for target protein are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove the entire unchained enzyme, a substrate solution which is acting on the bound enzyme is added to induce a colored reaction product.
Pancreatic amylase and salivary amylase are the intersections of assort genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Pancreatic amylase digests starch to maltose. The intensity of this colored product is directly proportional to the concentration of target protein demo incoming the samples.
DAI h-p-Amylase Quantitative provides a direct enzyme immunoassay incoming which pancreatic amylase embodies specifically acknowledged both by the solid phase anti-bodies and conjugate antibodies. This eliminates the interference of the added isoamylase and the problems derived from non-standardized substrates preparation and conditions that employed in most of the enzymatic assays.
More information: Pancreatic amylase ELISA kit
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