PDGF is synthesized mainly by megakaryocytes. It is stacked away fashionable the alpha granules of platelets from which it follows issued later on cellular phone activation of platelets for example by thrombin. There are two types of polypeptide, A (cardinal kDa, 124 amino acids) and barn (14 kDa, 140 amino acids), with about 50% sequence identity, disulfide linked into three affirmable dimeric motes, PDGF-AA, -abdominal muscle and –BB. PDGF binds to several plasma proteins and also to proteins of the extracellular intercellular substance. Inwards the grownup organism PDGF is involved in wound healing processes. The aberrant expression of PDGF follows discovered with vascular proliferative diseases so much as atherosclerosis. PDGF and PDGF-like factors are autocrine growth component since meningiomas.
In molecular biology, platelet-derived growth factor (PDGF) is one of the numerous growth factors, or proteins that regulate cell growth and division. In particular, it plays a significant role in blood vessel formation (angiogenesis), the growth of blood vessels from already existing blood vessel tissue. Uncontrolled angiogenesis is a characteristic of cancer. Chemically, PDGF is dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB).
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PDGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PDGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the 3 addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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