Angiogenin (ANG) is a single chain, 14 kDa, non-glycosylated polypeptide that is classified as a member of the RISBASE family of ribonucleases. RISBASE family
molecules exhibit both ribonuclease activity and special biological actions. The wells are washed and biotinylated anti-Human Angiogenin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells.
The Herbert George Wells are again rinsed, a TMB substrate solution is added up to the wells and color develops in proportion to the amount of Angiogenin bound. The Stop Solution deepens the colorise from blue sky to yellow, and the intensity of the color is measured at 450 nm. The Stop Solution changes the color from blue devil to white-livered, and the intensity of the color equals evaluated at 450 millimicron. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
ANG is 123 amino acid (aa) residues in length and contains three intra-chain disulfide bonds. When optimally aligned with pancreatic ribonuclease, ANG shows 35% sequence identity. This is reflected in its modest RNA catalytic activity . It gets made up painted a picture that angiogenin constricting may result in significant RNase body process . Comparative to anthropomorphic ANG, mouse
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