The human P-Cadherin Kit is a sandwich ELISA. The capture antibody is a polyclonal human P-Cadherin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known P-Cadherin concentration are added to these wells and appropriated to composite with the bound P-Cadherin antibody. This kit has been configured for research economic consumption exclusively and is not to be used in diagnostic or clinical procedures.
A biotinylated human P-Cadherin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB embodies expanded to induce a coloured reaction product.
The intensity of this colorised mathematical product constitutes immediately proportional to the concentration of P-Cadherin present in the tastes.
The determination from this kit embodies the in-vitro quantitative determination of human P-Cadherin in samples such as sera, plasma, tissue lysates and cell culture supernates.
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