Flt3 Ligand ELISA Kit (Enzyme-Linked Immunosorbent Assay) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Flt3 ligand in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Flt3 ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman Flt3 ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Flt3 Ligand is a ligand for the FLT3 tyrosine kinase receptor and belongs to a small group of growth factors that regulate proliferation of early hematopoietic cells. Multiple isoforms of Flt3 ligand have been identified. The predominant form is the transmembrane form, which is biologically active on the cell surface. When proteolytically cleaved the transmembrane isoform generates a soluble form, which is also biologically active. Flt3 ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3 ligand alone cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation.
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