Where to get Chlamydia Trachomatis IgG ELISA Kit?

Chlamydia Trachomatis IgG ELISA Kit is intended for the qualitative determination of IgG class antibodies against Chlamydia trachomatis in human serum or plasma (citrate). Chlamydia trachomatis IgG ELISA kit is a qualitative immunoenzymatic determination of IgG-class antibodies against Chlamydia trachomatis is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells of the Chlamydia trachomatis IgG ELISA kit are precoated with Chlamydia trachomatis antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured Chlamydia trachomatis -specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product using this Chlamydia trachomatis IgG ELISA kit is proportional to the amount of Chlamydia trachomatis -specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
The reagents of Chlamydia Trachomatis IgG ELISA Kit are stable up to the expiry date stated on the label when stored at 2...8 °C. The ready to use breakapart snap-off strips of this Chlamydia trachomatis IgG ELISA kit are coated with Chlamydia trachomatis antigen. Store at 2...8°C. Immediately after removal of strips, the remaining strips should be resealed in the aluminium foil along with the desiccant supplied and stored at 2...8 °C; stability until expiry date.
The Diagnostic Automation ELISA, Chlamydia Trachomatis IgG is intended for use in evaluating a patient's serologic status to Chlamydia Trachomatis infection. It is also used to evaluate paired sera for the presence of a significant increase in specific IgG as indicative of a recent or current Chlamydia Trachomatis infection.
Purified Chlamydia Trachomatis antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Chlamydia Trachomatis IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.
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