What is Cryptosporidium ELISA Kit?

Cryptosporidium ELISA Kit an in vitro immunoassay for the qualitative determination of Cryptosporidium antigen in feces. It is a double antibody (sandwich) ELISA using an anti-Cryptosporidium antibody to capture the antigen from the stool supernatant. A second anti-Cryptosporidium antibody is then added which sandwiches the captured antigen. This reaction is visualized by the addition of an antisecond antibody conjugated to peroxidase and the chromogen tetramethylbenzidine (TMB). The resulting blue color development indicates the presence of Cryptosporidium antigens being bound by the anti-Cryptosporidium antibodies.
Cryptosporidium is a coccidian parasite that is recognized as a strong enteric pathogen. This organism causes acute infection, even in people with normal immune systems. Cryptosporidium symptoms range from mild to severe diarrhea with a variety of complications.
The infection in people with comprised immune systems is much more severe and can be life-threatening. Passage of up to 12 liters per day of fluid has been reported.
Cryptosporidium infection can occur through several types of transmission. These include animal to human, water contamination, and person-to-person contact. Person-to-person contact may occur between members of the same household, day care centers, and homosexual men.
Cryptosporidum diagnosis was originally done by direct detection, using microscopic examination of stool samples using stains or fluorescent labeled antibodies as the most common procedures. This method relies on an experienced technician and observation of intact organisms. Historically, the method of microscopic examination has had poor proficiency in examinations. So more methods of diagnosis have been investigated.
Features of Cryptosporidium ELISA Kit
Results in less than 2 hours
Specific for Cryptosporidium oocyst antigen
Easy to perform
Highly standardized
High correlation with IFA-confirmed microscopy
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