What is Melatonin ELISA Kit?

Melatonin ELISA Kit is an enzyme immunoassay for the in-vitro diagnostic quantitative determination of melatonin in human serum and plasma. Melatonin ELISA kit assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the melatonin ELISA kit system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.
The melatonin ELISA kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sunlight. The storage and stability of specimen and prepared reagents is stated in the protocol insert. The microtiter strips of this melatonin ELISA kit are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C. After elution with methanol the Extraction Columns may be used for extraction of the next samples or stored at 2-8°C protected from dust. Extraction Columns may be re-used up to 4 times.
The results obtained using this melatonin ELISA kit themselves should not be the only reason for any therapeutical consequences. They have to be correlated to other clinical observations and diagnostic tests. A study with apparently healthy subjects using this melatonin ELISA kit has shown that the melatonin levels in humans have a marked circadian rhythmicity characterised by very low levels during day-time and high levels during night-time, and show a considerable inter-individual variation. Furthermore, the melatonin concentration is age dependent. The highest concentrations were found in samples of infants (up to 3 years). In a group of six healthy volunteers the circadian rhythm of melatonin was studied. The mean value reaches a minimum of about 4.6 pg/ml during daytime at 4 p. m. and a maximum of about 77.5 pg/ml during night time at 4 a.m. The nocturnal melatonin peak among healthy individuals varies significantly. It is recommended that each laboratory establishes its own range of normal values.
Melatonin has its highest levels in plasma during night time. Its characteristic nocturnal surge appears to encode temporal information such as length of night. Regulation of the melatonin secretion is under neural control. Sympathetic innervation seems to play a major role via its release of noradrenaline. Altered patterns and/or levels of melatonin secretion have been reported to coincide with sleep disorders, “jet lag“, depression, stress, schizophrenia, hypothalamic amenorrhea, pregnancy, anorexia nervosa, some forms of cancer, immunological disorders as well as control of sexual maturation during puberty. Most of the circulating melatonin is metabolized in the liver to 6-hydroxymelatonin and subsequently to 6- sulfatoxymelatonin which is excreted into the urine.
Melatonin ELISA Kit is a Solid phase ELISA based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the we
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