Anti-OVA allows for the specific measurement of Guine-pig Anti-OVA-IgE concentrations in cell culture supernates, serum, and plasma.
Introduction
Measurement of anti-OVA-IgE levels after OVA-inoculation to mouse will be helpful in clarification of the immune process.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. OVA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Anti-OVA-IgE present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Anti-OVA-IgE is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-OVA-IgE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Ovalbumin (OVA) is widely used as an antigen for studying allergic diseases in mice. Chondrex provides three types of mouse IgE and one type of IgG anti-OVA antibody assay kit. These IgE assay kits employ an identical IgE standard (Clone E-C1), which allows for easy determination of total IgE and OVA specific IgE ratios in individual samples.
In addition, rat anti-mouse IgE monoclonal antibodies (Clone 345-2 and 77-1) used in these kits react to both IgEa and IgEb allotypes. Therefore, separate assays for IgEa and IgEb allotypes are not required.
This kit is designed to simply determine anti-OVA IgE levels using an OVA-coated ELISA plate for samples used for in vitro experiments, such as an IgE solution used for stimulating mast cells. This ELISA kit includes reagents to measure 40 samples. The assay range is approximately 0.8-50 ng/ml.
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