1. Bring all kit components and samples to room temperature (18-25 ℃) before use.
2. Dispense 10 uL of LYSIS BUFFER SOLUTION into 100 uL specimens, mix and stand for one hour (The proportion of LYSIS BUFFER and Specimens should be no less than 1:10). (NOTE: This step is required when the sample is cell culture fluid & body fluid & tissue homogenate; if the sample is serum or blood plasma, then this step should be skipped.)
3. Wash Solution - Dilute 10 mL of Wash Solution concentrate (100×) with 990 mL of deionized or distilled water to prepare 1000 mL of Wash Solution (1×).
Application ELISA
Storage of Endotoxin ELISA Kit
All kit capacity can be stored at 2-8 degree. From a user angle this is added acceptable if it comes to carriage and accumulator than a claim for freezing some reagents, as accepted for abounding added ELISA kits.
Reliability
Thanks to just one incubation- and abrasion footfall the Elisa agreement can be performed aural two hours. Compared to the acceptable Elisa method, beneath administration accomplish abate errors and bear added constant results. Thorough and approved tests of the arrangement agreement a abundant after and inter appraisal believability and ensure a low accessory of variation.
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