Specifications of Follistatin ELISA Kit

Follistatin ELISA Kit
Tests: 1 x 96 well plate
Sample type
Cell culture supernatant, Urine, Serum, Plasma
Assay type: Sandwich
Sensitivity: < 0.5 ng/ml
Range: 0.512 ng/ml - 125 ng/ml
Recovery: 90%
Synonyms: CG12955, CG12956, Fol1, dFS, dFol1, Dmel\CG33466, CG33466, FS, xfs, MGC79635, FST, fst, LOC100033825, Fst, AL033346, FOL1, Fst-288, RATFOL1
Follistatin ELISA Kit(Enzyme-Linked Immunosorbent Assay) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Follistatin in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Follistatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Follistatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Follistatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Follistatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage    
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C . Avoid repeated freeze-thaw cycles.
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