This immunoassay kit allows for the specific measurement of human secreted phospholipase A2, sPLA2 concentrations in cell culture supernates, serum and plasma.
Introduction
Phospholipase A2 (PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid. The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell.
Test principle
sPLA2 ELISA Kit employs the quantitative sandwich enzyme immunoassay technique. A antibody specific for sPLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sPLA2 present is bound by the immobilized antibody. An enzyme-linked antibody specific for sPLA2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sPLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Description
This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin and mucous membranes. In case of contact, rinse affected area with plenty of water. Observe all federal, state and local regulations for disposal.
All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.
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