Anti-CCP, which stands for anti-cyclic citrullinated peptide antibody, is a new and exciting blood test to help doctors confirm a diagnosis of rheumatoid arthritis. Rheumatoid Arthritis (RA), a chronic inflammatory disorder of the synovial membranes, is one of the most common systemic autoimmune diseases. This immunoassay kit allows for the in vitro quantitative determination of human anti-CCP concentrations in serum, plasma and other biological fluids. Early diagnosis of RA is important both in disease treatment and management. Approximately 1% of the world population is affected. The diagnosis of RA depends primarily on clinical manifestations, but laboratory results are helpful in differential diagnosis and disease management.
Historically, rheumatoid factor (RF) has long been the serologic indicator for RA. However, it has been known for years that anti-keratin autoantibodies (AKA), also known as anti-perinuclear autoantibodies, are detected in 40-55% of RA patients and in 40-50% of clinically diagnosed RA patients who are RF negative.
Specification: 96test.
How to Use CCP ELISA Kit
When mixing or reconstituting protein solutions, always avoid foaming. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions.
2.used to separate reservoirs for each reagent. When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.
3.To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Substrate Solution should remain colorless until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless to gradations of blue.
4.Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.
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