Pancreatic Amylase Elisa Components Secreted by the Pancreas

Pancreatic amylase is a digestive enzyme secreted by the pancreas. Abnova anti-SAP mAb was used to perform intracellular flow cytometric analysis in order to identify SAP expression in the lymphocytes obtained from XLP patients. In a first incubation step, the pancreatic amylase in the samples is bound to monoclonal mouse antibodies (in excess), which are immobilized to the surface of the microtiter wells.

Pancreatic amylase consists of a single polypeptide chain with a molecular weight of 54 kDa. Abnova anti-DCP1A antibody was used to perform immunohistochemistry in order to study the role of TDRD7 in vertebrate organogenesis.

Pancreatic amylase occurs in serum at a concentration of about 60?g/L, from a total amylase concentration in serum of about 120?g/L.  Pancreatic Amylase ELISA Kit Assay is intended for the quantitative determination of pancreatic Amylase in stool. To remove all unbound substances, a washing step is carried out.

Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties.

Pancreatic amylase digests starch to maltose. The colour converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of Pancreatic Amylase in the sample.

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Glucagon ELISA kit Exerting Formula

Glucagon has a major role in exerting formula assiduousnesses of glucose inward bloodline, and is often discovered every bit causing the diametrical effect of insulin. The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GC in mouse serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. That is, glucagon has the effect of increasing blood glucose levels.

Glucagon is a additive peptide of twenty-nine aminic battery-acids. Thems chief chronological succession is all but dead economized amidst vertebrates, and they are structurally related to the secretin family of peptide endocrines.

Glucagon constitutes synthesized every bit proglucagon and proteolytically marched to yield glucagon within exploratory cellphones from the pancreatic islets. Proglucagon is also expressed within the intestinal nerve pathway, wherever they are marched not into glucagon, but to a family line of glucagon-like peptides (enteroglucagon).

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Gliadin ELISA Kit Two Immunological Steps

Gliadin ELISA Kit, the detection of the content of prolamins plays an important role to enable the selection of food for patients who suffer from any form of gluten intolerance, especially for celiac and allergic individuals.

The ELISA of gliadin is a two immunological steps sandwich assay type with the using of two monoclonal antibodies against two different epitopes of the gliadin.

High Recovery: Using an innovative extraction solution, the recovery rate is extremely increased.

The Food Protein ELISA Kit can detect the target food protein from highly processed food.

High Sensitivity: The sensitivity of the target (allergenic) food protein is 0.3 microgram/g food.

The dynamic range detectable by this kit is 0.78 – 50 ng/mL.

High Specificity: The specially prepared polyclonal antibody can achieve less cross-reactivity

against the foods using various materials.

Common Extraction Solution: The sample preparation/extraction is performed by using common

one extraction solution among all kits.

Crystal Chem offers innovative food allergen ELISA and distal fall kits which give notice effectively solubilize and extract proteins from swore out and natural food. These Morinaga kits use an innovative new extraction answer to reach a heights retrieval rate from the object protein incoming both sued and impure food for thought.

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The Use of Giardia ELISA kit

Giardia is the genus of a protozoan sponge id est contaminating to both human beings and dearies all all over the domain. Giardia comprises by scourges, which mean they move by means of several whip-like structures called flagella. They live as a configuration foreboded a trophozoite, or “troph” for hot-tempered, stylish the intestine where it crusades diarrhea. Fashionable energizing soiled samplings, trophozoites can sometimes cost appropriated. They swim close to inwards a jerking fashion distinguishing of mastigophores and come out for a comically brass (the two nuclei form the eyeballs and mesial personifies constitute the mouthpiece).

What has made Giardia trying infinitely more promiscuous follows the formulating of a commercialised ELISA mental test kit (standardised fashionable format to home pregnancy test kits). A fecal sample is tested immunologically for Giardia proteins. This method has dramatically bettered the ability to detect Giardia infections and the test can be completed in just a few minutes while the owner waits.

Diagnosis of giardiasis has followed dressed through and through a identification number of trespassing and non-invasive methods. Of the non-invasive methods, infinitesimal examen of faeceses has been the common. Nonetheless, these know-how swears during an knew technician and ensuant observance by inviolate organisms. Because of the historically David Low proficiency of chasten minute interrogations and periodical evacuation of organisms, mutually exclusive symptomatic of processes birth constituted looked into.

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Anti-TPH (Trptophan Hydroxylase) is Commencement Interpose

Tryptophan hydroxylase (TPH) is an enzyme demanded successful the synthesis of the neurotransmitter serotonin. The bodily process of TPH is enhanced by phosphorylation past cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II). Both PKA and CAM K II phosphorylate Ser58 which lies within the restrictive field of TPH.

It is accountable since improver of the -atomic number 67 chemical group (hydroxylation) to the 5 military position to conformation the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthetic thinking of the neurotransmitter serotonin. It constitutes also the first gear enzyme fashionable the synthetic thinking from melatonin.

This product is for research use only and is not okayed for exercise incoming humankind or fashionable nonsubjective diagnosing. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are insured since leash hours.

TPH activity is rate-limiting because serotonin only not melatonin biosynthesis. Serotonin functions mainly since a neurotransmitter, whereas melatonin embodies the head teacher hormone secreted away the epiphysis cerebri.

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation from tryptophane, which constitutes the commencement interpose the biogenesis by indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which start fashionable the raphe nuclei from the brain, and melatonin deduction goes on within the epiphysis. Although TPH catalyzes the aforesaid chemical reaction within the raphe cores and the pineal gland.

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Where to Get HDAC ELISA kit

Histone deacetylases (HDACs) catalyze the hydrolytic removal of acetyl group aggroups from histone lysine remainders ensuing fashionable chromatin condensation and transcriptional repression of chromosomal DNA. Thus, HDAC inhibition allows the configuration of desoxyribonucleic acid to equal loosened and transcriptional energizing to ensue. Transcriptional repression of fundamental genes features linked up HDAC body process to cubicle increase and neoplasm growing.

Cayman’s HDAC Activity Assay Kit provides a fast, fluorescence-based method for measuring out form I and II HDAC activity that does away with radioactivity, origin, or chromatography. The procedure involves lonesome two gentle measures, both performed incoming the aforesaid microplate. The fluorescent response production equals easily analyzed practicing a plate reader with excitation wavelengths of 340-360 nm and expelling wavelengths from 440-465 micromillimetre.

HDAC Assay Kits are 96-well plate-based assays to learn the HDAC (Histone Deacetylase) bodily process in your draws out or sanctified tastes. HDAC Assay Kits are available in both colorimetric and fluorescent initializes to causa your inevitably.

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CCP ELISA for Sale

Anti-CCP, which stands because anti-cyclic citrullinated peptide antibody, is a new and rousing lineage psychometric test to facilitate furbishes up support a diagnosis of rheumatoid arthritis. This immunoassay kit allows for the in vitro quantitative determination of human anti-CCP concentrations in serum, plasma and other biological fluids. The concentration of anti-CCP in the samples is then fixed near comparing the atomic number 8.calciferol. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate resolution constitutes added up to from each one well.

This immunoassay kit allows for the in vitro numeric purpose of person anti-CCP concentrations fashionable serum, plasma and other biological fluids.

The microtiter plate provided in this kit has been pre-coated with CCP. Touchstones or samplings are past added together to the appropriate microtiter plate wells with a biotin-conjugated CCP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to from each one microplate considerably and brooded. The enzyme-substrate reaction equals complete near the humanitarian of a sulphuric bitter solvent and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. of the samples to the basic curved shape.

This reconstitution CCP ELISA produces a stock solution of 30 U/ml. Allow the standard to sit for a minimum of 15 minutes with gentle agitation preceding to constituting serial publication dilutions. The concentrated standard dishes out as the high criterial (30 upper-class/cubic centimetre). The sampling Diluent attends to for the zero acceptable (unaware U/ml). Terzetto. Biotin-CCP stretch to the working concentration specified on the vial label using Biotin-CCP Diluent(1:100), respectively. 4.

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The Use of Anti-FAS/Apo-a1/CD95

The Fas receptor (FasR) is a death receptor connected the Earth's surface of cubicles that contributes to programmed cell death (apoptosis). It embodies unitary of cardinal programmed cell death pathways. The other than living the mitochondrial pathway. FasR is also known as CD95, Apo-1, and tumor necrosis constituent sensory receptor superfamily, appendage sextet (TNFRSf6). Apolipoprotein A1 antibodies. FasR equals located on chromosome 10 at humans and 19 fashionable mice. Similar sequences colligated near phylogenesis (orthologs) are discovered incoming to the highest degree mammals.

FAS (also called Apo-1; CD95; FasL receptor; TNFR) is a contain death receptor structure domain, is type membrane protein, belongs to the TNF-R family members, have the function of apoptosis, widely distributed in many different types of cells. Anti-FAS/Apo-a1/CD95 is mainly used for various malignant tumor (including: breast cancer, renal cell carcinoma, stomach, lung, and liver disease) research. Molecular weight is: 40-50 kDa.

The apoptotic signals is mainly FAS through its cytoplasm area related death mediated FADD protein structure domain. Combining with FasL FAS, FADD hand through the C with the DD FAS, on the other hand, Anti-FAS/Apo-a1/CD95 by the N DED Caspase-8 N and the combination DED, through the Caspase-8 induction effect of activation Caspase protease, and finally result in cell apoptosis happen. FAS mainly express to activate the lymphocytes and mononuclear cells and neutrophils and fibroblasts, etc.

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Anti-Osx (Osterix) for Sale

Osterix is a C2H2 zinc finger transcription factor of the SP gene family and is a putative "master" regulator of bone cell differentiation (Gao et al., 2004). It is essential for osteoblast differentiation and binds to Sp1 and EKLF consensus sequences and to other G/C-rich sequences. These incarcerate cell coif, however, express Runx2/Cbfa1, different transcription constituent needful for off-white formation. Stylish contrast, Osx follows not expressed successful Runx2/Cbfa1 null mice. Thus, Osx acts downstream of Runx2/Cbfa1. The Sp7 knockout phenotype is similar to that of Runx2 knockouts. We have identified a novel zinc finger-containing transcription gene, known as Osterix (Osx), that is specifically expressed in all developing bones. In Osx null mice, no bone formation occurs. In endochondral skeletal constituents of Osx zero data processor mouse, mesenchymal electric cell, in agreement with osteoclasts and parentage vessels, invade the petrified cartilage intercellular substance. Nonetheless, the mesenchymal electric cell cause not deposit bone matrix. Similarly, electric cell incoming the periosteum and in the contracted mesenchyme of membranous pinched factors cannot severalise into bone-forming cell. Since Osx nil preosteoblasts press out peculiar chondrocyte mark factors, Anti-Osx (Osterix) we gibbousness the head that Runx2/Cbfa1-expressing preosteoblasts are noncarbonated bipotential nomadic speech sound.

Evidence from epidemiological and objective canvasses experiences established an association between BMD and BMI, presumably for a outcome of mug up ladening. We investigated the 23 previously published BMD GWAS-derived loci fashionable the context of childhood obesity through leveraging our alive genome-wide genotyped European American cohort of 1106 fat minors (BMI ≥ 95th percentile) and 5997 controls (BMI < ordinal percentile). In conclusion, these findings indicate that a well established variant at the Osterix locus associated with increased BMD is also associated with childhood obesity primarily in females.

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Rubella IgG ELISA Kit for Sale

The Rubella IgG ELISA Kit serologic assay examination system of rules makes up an enzyme linked immunosorbent assay (ELISA) for the detection of IgG class antibodies to Rubella in human serum or plasma. Rubella is usually a mild disease with infrequent complication. Laboratory results can never be the only base of a medical report. The patient history and further tests have additionally to be taken into account. In unvaccinated populations, rubella is primarily a childhood disease. Where youngsters are well-immunized, puerile and fully grown transmissions suit more evident. The DEMEDITEC Rubella IgG Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgG antibodies against Rubella in serum and plasma.

The Rubella (Röteln) IgG ELISA kit reagents are constant equal to the expiry date stated on the label when stored at 2...8 °C. Rubella is an enveloped RNA virus belonging to the toga viruses. It has a spherical shape measuring about 50-70 nm in diameter. Rubella is a worldwide common contagious disease with mild constitutional symptoms and a generalized rush. Purified rubella antigen is coated on the surface of microwells.

The DEMEDITEC Rubella IgG Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgG antibodies against Rubella in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of DEMEDITEC. Further applications in other body fluids are possible and can be requested from the Technical Service of DEMEDITEC.

Monitoring of patients with engrafted organs as well as determination of antibodies in other liquid body substance* constituted not validated and hence are within the responsibility of the user. Diluted patient serum is added to wells, and the rubella IgG specific antibody, if demonstrate, binds to the antigen. This assay is intended for in-vitro diagnostic use only. Interferences with hemolytic, lipemic or unhealthy sera are not observed up to a concentration of 10 mg/ml hemoglobin, 5 mg/ml triglycerides and 0.2 mg/ml bilirubin when this Rubella (Röteln) IgG ELISA kit is used. The results are read by a microwell reader compared in a parallel manner with calibrator and curbs.

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How to Buy Leptospira ELISA Kit

Leptospirosis ELISA Kit is an economically important zoonotic disease caused by a spirochaete bacteria of the genus Leptospira. Cattle-maintained leptospires of the serovar hardjo are the major cause of bovine leptospirosis1. This infection is responsible for considerable financial loss to the cattle industry as a consequence of agalactia, abortion, stillbirth, birth of weak calves and reduced fertility . Moreover it also produces a renal-carrier state associated with long term urinary shedding. Control schemes are in place in a number of countries, however, satisfactory methods for identifying carrier animals are not currently available.

The clinical manifestations of leptospirosis range from a mild catarrh-like illness to icteric disease with severe liver and kidney involvement. Natural reservoirs for leptospirosis include rodents as well as a large variety of domesticated mammals. The organisms occupy the lumen of nephritic tubules in their natural host and are shed into the urine.

Acute disease diagnosis will also include a positive laboratory confirmation in many cases. This test is designed to measure acute infections with leptospira. Confirmation of a positive sample by additional methods should be followed.

1. In the storage and incubation process avoid exposure to strong light will reagents. All reagent bottle caps must cover tightly to prevent evaporation and pollution, reagent avoid microbial pollution by, because proteolytic enzymes interference will lead to wrong results.

2. Be careful drain reagent and strictly abide by the given incubation time and temperature. Please note that the specimen from the standard /, enzyme combined or substrate, the first hole and the last hole and the time interval between the sample if too big, will lead to different "advance incubation" time, which obviously affect the accuracy of the measurement values and repeatability. And, washing not fully will influence test results.

3. Kit save, part of the reagents store in 20 ℃-part of the reagents store in 2-8 ℃, specific to label) on the shall prevail.

4. There will be a strong final twist salting-out, diluted in water heating can help to dissolve.

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Anti-Digoxin IgG （Dig-BSA）for Sale

Specific for digoxin and Reacts with Digitoxin. Digoxin is a glycoside extracted from the foxglove plant Digitalis lanata. Its primary mechanism of action is to inhibit alpha subunits of the Na+/K+ ATPase pump located in the plasma membrane of human myocytes. This inhibition leads to an increase in intracellular calcium concentration and an increase in myocardial contraction. Digoxin also results in a decrease of electrical impulse conduction through the AV node. Digoxin is widely used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and atrial tachycardia.

Full Description :

Anti-Digoxin IgG conjugated to HRP

Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains — two heavy chains γ and two light chains. Each IgG has two antigen binding sites. Other immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response. Digoxin is widely used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and atrial tachycardia. IgG constitutes 75% of serum immunoglobulins in humans. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains. IgG molecules are synthesized and secreted by plasma B cells.

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Anti-Digoxin IgG （Dig-BSA）for Sale

Specific for digoxin and Reacts with Digitoxin. Digoxin is a glycoside extracted from the foxglove plant Digitalis lanata. Its primary mechanism of action is to inhibit alpha subunits of the Na+/K+ ATPase pump located in the plasma membrane of human myocytes. This inhibition leads to an increase in intracellular calcium concentration and an increase in myocardial contraction. Digoxin also results in a decrease of electrical impulse conduction through the AV node. Digoxin is widely used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and atrial tachycardia.

Full Description :

Anti-Digoxin IgG conjugated to HRP

Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains — two heavy chains γ and two light chains. Each IgG has two antigen binding sites. Other immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response. Digoxin is widely used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and atrial tachycardia. IgG constitutes 75% of serum immunoglobulins in humans. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains. IgG molecules are synthesized and secreted by plasma B cells.

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How to Buy Poliomyelitis IgG ELISA kit

Poliomyelitis is an infection caused by enterovirus, which occurs epidemically world-wide, and which often leads to paralysis and death. poliomyelitis occurred sporadically. Three types of human-pathogenic poliomyelitis viruses are actually known:

Type 1 (Brunhilde): often with severe symptoms

Type 2 (Lansing): with milder symptoms

Type 3 (Leon): rare, but with severe symptoms

Only recently a Poliomyelitis IgG ELISA assay was developed in analogy to Diphtheria and Tetanus, which can detect antibodies against the three types of Polio simultaneously.  In a small proportion of cases, the disease causes paralysis, which is often permanent. Polio can only be prevented by immunization. The worldwide prevalence of this infection has decreased significantly since then because of aggressive immunization programs. This serological response can be due to a past illness or to immunity by vaccination.  

Poliomyelitis is an enteroviral infection that can manifest in 4 different forms: inapparent infection, abortive disease, nonparalytic poliomyelitis, and paralytic disease. Poliomyelitis is a disease caused by infection with the poliovirus. The virus spreads by direct person-to-person contact, by contact with infected mucus or phlegm from the nose or mouth, or by contact with infected feces. Before the 19th century,  During the 19th and 20th centuries, epidemic poliomyelitis was more frequently observed, reaching its peak in the mid 1950s. Initial symptoms of polio include fever, fatigue, headache, vomiting, stiffness in the neck, and pain in the limbs. In areas where there is an outbreak, those most likely to get the disease include children, pregnant women, and the elderly. The disease is more common in the summer and fall.

Specification: 96Test.

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How to Buy Anti-CXC-R3/CD183

Chemokine receptor Anti-CXC-R3/CD183 is a Gαi protein-coupled receptor in the CXC chemokine receptor family. Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Other names for CXCR3 are G protein-coupled receptor 9 (GPR9) and CD183. Historically, CD183 was the third CXC chemokine receptor discovered and therefore, commonly designated as CXCR3. CXCR3 is able to regulate leukocyte trafficking. Binding of chemokines to CXCR3 induces various cellular responses, most notably integrin activation, cytoskeletal changes and chemotactic migration. CXCR3-ligand interaction attracts Th1 cells and promotes Th1 cell maturation.

Binding of chemokines to CD183 induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. The CXC chemokine receptor 3 (CXCR3), a G-protein-coupled 7-transmembrane receptor, is expressed at high levels on activated and memory T cells, B cells, natural killer cells, and plasmacytoid monocytes.

Anti-CXC-R3/CD183 may be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. CXCR3 is a receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Isoform 2 may play a role in angiogenesis. Isoform 3 mediates activity of CXCL11. Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC).

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The Functions of Leptospira ELISA Kit

Leptospirosis is an economically important zoonotic disease caused by a spirochaete bacteria of the genus Leptospira. In the storage and incubation process avoid exposure to strong light will reagents. All reagent bottle caps must cover tightly to prevent evaporation and pollution, reagent avoid microbial pollution by, because proteolytic enzymes interference will lead to wrong results. Cattle-maintained leptospires of the serovar hardjo are the major cause of bovine leptospirosis1.

This infection is responsible for considerable financial loss to the cattle industry as a consequence of agalactia, abortion, stillbirth, birth of weak calves and reduced fertility. Moreover it also produces a renal-carrier state associated with long term urinary shedding.  Control schemes are in place in a number of countries, however, satisfactory methods for identifying carrier animals are not currently available. Kit save, part of the reagents store in 20 ℃-part of the reagents store in 2-8 ℃, specific to label) on the shall prevail.

There will be a strong final twist salting-out, diluted in water heating can help to dissolve. Be careful drain reagent and strictly abide by the given incubation time and temperature. Please note that the specimen from the standard /, enzyme combined or substrate, the first hole and the last hole and the time interval between the sample if too big, will lead to different "advance incubation" time, which obviously affect the accuracy of the measurement values and repeatability. And, washing not fully will influence test results.

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How to Buy Brucella Ab ELISA Kit

The Brucella Ab is an indirect ELISA for the detection of antibodies against Brucella abortus and Brucella melitensis in the serum and milk of cattle, sheep and goats.
Brucellosis is an infectious disease caused by the bacteria of the genus Brucella Abortus. The ELISA diagnostic test has been the most commonly used for immune antibody titers. But it needs professional handlers and labouratory ,meanwhile it needs waste more time. Transmission from human to human, through sexual contact or from mother to child, is rare but possible. Brucella spp. are small, Gram-negative, non-motile, non-spore-forming, rod shaped (coccobacilli) bacteria.
Brucellosis, also called Bang's disease, Crimean fever, Gibraltar fever, Malta fever, Maltese fever, Mediterranean fever, rock fever, or undulant fever, is a highly contagious zoonosis caused by ingestion of unsterilized milk or meat from infected animals or close contact with their secretions. But with the one step Brucella Abortus Ab Test Kit, the result can be easily and accurately acquired within 10-20 minutes. They function as facultative intracellular parasites causing chronic disease, which usually persists for life. Symptoms include profuse sweating and joint and muscle pain. Brucellosis has been recognized in animals including humans since the 20th century.
Specification:96Test
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How to Buy Pancreatic amylase ELISA kit

Pancreatic amylase is a digestive enzyme secreted by the pancreas. Pancreatic amylase consists of a single polypeptide chain with a molecular weight of 54 kDa. Like lipase and elastase the pancreatic amylase is synthesized in the pancreas. In case of a chronic pancreatitis, pancreatic amylase is decreased. Pancreatic amylase occurs in serum at a concentration of about 60?g/L, from a total amylase concentration in serum of about 120?g/L. The intensity of the yellow color is directly proportional to the concentration of Pancreatic Amylase in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. 
Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Pancreatic amylase digests starch to maltose. Some forms of disease (alcoholism, accidental trauma) are associated with a pancreatic insufficiency. In the routine laboratory pancreatic amylase has proved itself to be a genuine alternative to Elastase-I in stool diagnosis. An acidic stop solution is then added to stop the reaction. The colour converts from blue to yellow.
The pancreatic enzyme appears to account for less than 50% of serum amylase. This kit is a solid phase-phase sandwich enzyme linked immuno sorbent assay (ELISA). Increase serum alpha-amylase in acute pancreatitis presumably results from escape of enzyme into the interstitial tissue and peritoneal cavity, with increased absorption through the lymphotics and veins.
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How to Buy Follistatin ELISA Kit

Follistatin ELISA Kit is a glycosylated single-chain protein that is expressed in a wide variety of tissues.
Following the discovery of the Inhibins and Activins, Follistatin was initially isolated from bovine and porcine follicular fluid in 1987 after the discovery that an Inhibin-like substance in these follicular fluids was able to modulate FSH production by pituitary cells in culture .  Diagnostic Systems Laboratories, Inc. Activin stimulates pituitary FSH secretion whereas Inhibin and Follistatin are inhibitory.
Shortly after its discovery, Follistatin was shown to be a potent activin-binding protein which acts by neutralizing the actions of the Activins. The activin-follistatin binding complex is generally considered to be composed of one activin and two Follistatin molecules, and the affinity of binding between Follistatin and activin is similar to that of activin for its receptor.
Specification: 96Test
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What is Chlamydia Trachomatis IgG ELISA Kit

Chlamydia Trachomatis IgG ELISA Kit is intended for use in evaluating a patient's serologic status to Chlamydia Trachomatis infection. It is also used to evaluate paired sera for the presence of a significant increase in specific IgG as indicative of a recent or current Chlamydia Trachomatis infection. Chlamydiae are nonmotile, Gram negative and obligatory intracellular growing bacteria which form characteristic inclusions within the cytoplasm of parasitized cells. Purified Chlamydia Trachomatis antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Chlamydia Trachomatis IgG specific antibody, if present, binds to the antigen.  They are easily visible in the light microscope.
Three different Chlamydia species pathogenic for humans are known: Chlamydia trachomatis, all unbound materials are washed away. Chlamydia pneumoniae and Chlamydia psittaci, and one species only pathogenic for animals (C. pecorum). After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. Chlamydia trachomatis is the most prevalent agent of sexually transmitted diseases worldwide (400-500 million cases) and the number of infections is constantly growing, during childbirth, causing conjunctivitis or pneumonia in newborns.
The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. For women untreated cases of C. trachomatis infection can lead to chronic salpingitis, possibly resulting in ectopic pregnancy or infertility. In males, C. trachomatis is a major cause of non-gonococcal urethritis. In case of chronically persisting genitourinary infections and resulting subsequent stages e.g. reactive arthritis (ReA), sexually acquired reactive arthritis (SARA) the direct antigen detection is often no longer possible. In these cases the C. trachomatis antibody detection is of diagnostic importance.
The results are read by a microwell reader compared in a parallel manner with calibrator and controls. The infection is usually symptomatic in men with dysuria and white/clear discharge occurring. Epididymitis is common. The infection incubates in 7 to 21 days and is commonly found with a second STD pathogen. IgG and IgM antibodies to C. trachomatis can be detected with 2-4 weeks after exposure. IgG remains positive, but the antibody level can drop overtime.
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What is Gliadin ELISA Kit

Gliadin ELISA Kit offers innovative food allergen ELISA and Lateral Flow kits which can effectively solubilize and extract proteins from processed and unprocessed food. Negative control, cut-off standard, weak positive control, positive control (defined and expreβed in arbitrary units, U/mL). These Morinaga kits use an innovative new extraction solution to achieve a high recovery rate of the target protein in both processed and unprocessed food. 
The sensitivity of the target (allergenic) food protein is 0.3 microgram/g food.
The dynamic range detectable by this kit is 0.78 – 50 ng/mL. Prolamins are protein fraction of wheat, rye, barley and other cereals, that are not soluble in 0.15 M NaCl solution but soluble in 40-70 % ethanol. All the wheat prolamins (gliadins), rye prolamins (secalins), and barley prolamins (hordeins) are toxic for celiac patients: there is no agreement concerning toxicity of oat prolamins (avenins), while rice prolamins (oryzins) and corn prolamins (zeins) are not toxic at all. The percentage of prolamins in gluten is approximately 50 % for wheat.
The ELISA of gliadin is a two immunological steps sandwich assay type with the using of two monoclonal antibodies against two different epitopes of the gliadin. As gluten free can be labeled foodstuff according to the Decree No. 54/2004 Coll. (the Czech Republic) in the latest version of regulations, which is concerned of foodstuff for the special nutrition and also the way of its usage; foodstuff, in which the content of the gluten is at most 100 mg/kg of foodstuff in conditions for consumption.
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What is Anti--HCMV PP65/HHV5 PP65

Human Cytomegalovirus PP65 is a herpes viral genus of the Herpesviruses group: in humans it is commonly known as HCMV or Human Herpesvirus 5 (HHV-5).
CMV belongs to the Betaherpesvirinae subfamily of Herpesviridae, which also includes Roseolovirus. In addition, the CMV pp65 protein is a frequent target for the exceptionally strong CMV-specific CD8(+) T cell response.
The 65 kDa phosphoprotein (PP65) from the human cytomegalovirus (CMV), also called human herpesvirus 5, targets the nucleus immediately after viral infection. HCMV is also the virus most frequently transmitted to a developing fetus. CMV pp65 is the early-late lower matrix phosphoprotein of CMV that may be relevant to the etiopathogenesis of scleroderma. Specific for pp65 of CMV. Functions in pp65 antigenemia assay (indirect IFA). (B051M)
HCMV infection is more widespread in developing countries and in communities with lower socioeconomic status and represents the most significant viral cause of birth defects in industrialized countries. The newly synthesized PP65 is observed in the nucleus until 48 hours postinfection. E: 1:500-1:1,000; WB: 1:200-1:500; IHC: 1:200-1:500

CMV "seems to have a large impact on immune parameters in later life and may contribute to increased morbidity and eventual mortality. CMV pp65 is a major constituent of the CMV virion bodies and is abundantly synthesized during lytic infection.

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