What is Chlamydia Trachomatis IgG ELISA Kit

Chlamydia Trachomatis IgG ELISA Kit is intended for use in evaluating a patient's serologic status to Chlamydia Trachomatis infection. It is also used to evaluate paired sera for the presence of a significant increase in specific IgG as indicative of a recent or current Chlamydia Trachomatis infection. Chlamydiae are nonmotile, Gram negative and obligatory intracellular growing bacteria which form characteristic inclusions within the cytoplasm of parasitized cells. Purified Chlamydia Trachomatis antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Chlamydia Trachomatis IgG specific antibody, if present, binds to the antigen.  They are easily visible in the light microscope.
Three different Chlamydia species pathogenic for humans are known: Chlamydia trachomatis, all unbound materials are washed away. Chlamydia pneumoniae and Chlamydia psittaci, and one species only pathogenic for animals (C. pecorum). After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. Chlamydia trachomatis is the most prevalent agent of sexually transmitted diseases worldwide (400-500 million cases) and the number of infections is constantly growing, during childbirth, causing conjunctivitis or pneumonia in newborns.
The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. For women untreated cases of C. trachomatis infection can lead to chronic salpingitis, possibly resulting in ectopic pregnancy or infertility. In males, C. trachomatis is a major cause of non-gonococcal urethritis. In case of chronically persisting genitourinary infections and resulting subsequent stages e.g. reactive arthritis (ReA), sexually acquired reactive arthritis (SARA) the direct antigen detection is often no longer possible. In these cases the C. trachomatis antibody detection is of diagnostic importance.
The results are read by a microwell reader compared in a parallel manner with calibrator and controls. The infection is usually symptomatic in men with dysuria and white/clear discharge occurring. Epididymitis is common. The infection incubates in 7 to 21 days and is commonly found with a second STD pathogen. IgG and IgM antibodies to C. trachomatis can be detected with 2-4 weeks after exposure. IgG remains positive, but the antibody level can drop overtime.
More about: Buy Chlamydia Trachomatis IgG ELISA Kit
Read more>> Elisa assay kits