Pancreatic amylase is a digestive enzyme secreted by the pancreas. Pancreatic amylase consists of a single polypeptide chain with a molecular weight of 54 kDa. Like lipase and elastase the pancreatic amylase is synthesized in the pancreas. In case of a chronic pancreatitis, pancreatic amylase is decreased. Pancreatic amylase occurs in serum at a concentration of about 60?g/L, from a total amylase concentration in serum of about 120?g/L. The intensity of the yellow color is directly proportional to the concentration of Pancreatic Amylase in the sample. A dose response curve of the absorbance unit (optical density, OD) vs.
Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Pancreatic amylase digests starch to maltose. Some forms of disease (alcoholism, accidental trauma) are associated with a pancreatic insufficiency. In the routine laboratory pancreatic amylase has proved itself to be a genuine alternative to Elastase-I in stool diagnosis. An acidic stop solution is then added to stop the reaction. The colour converts from blue to yellow.
The pancreatic enzyme appears to account for less than 50% of serum amylase. This kit is a solid phase-phase sandwich enzyme linked immuno sorbent assay (ELISA). Increase serum alpha-amylase in acute pancreatitis presumably results from escape of enzyme into the interstitial tissue and peritoneal cavity, with increased absorption through the lymphotics and veins.
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