Leptospirosis ELISA Kit is an economically important zoonotic disease caused by a spirochaete bacteria of the genus Leptospira. Cattle-maintained leptospires of the serovar hardjo are the major cause of bovine leptospirosis1. This infection is responsible for considerable financial loss to the cattle industry as a consequence of agalactia, abortion, stillbirth, birth of weak calves and reduced fertility . Moreover it also produces a renal-carrier state associated with long term urinary shedding. Control schemes are in place in a number of countries, however, satisfactory methods for identifying carrier animals are not currently available.
The clinical manifestations of leptospirosis range from a mild catarrh-like illness to icteric disease with severe liver and kidney involvement. Natural reservoirs for leptospirosis include rodents as well as a large variety of domesticated mammals. The organisms occupy the lumen of nephritic tubules in their natural host and are shed into the urine.
Acute disease diagnosis will also include a positive laboratory confirmation in many cases. This test is designed to measure acute infections with leptospira. Confirmation of a positive sample by additional methods should be followed.
1. In the storage and incubation process avoid exposure to strong light will reagents. All reagent bottle caps must cover tightly to prevent evaporation and pollution, reagent avoid microbial pollution by, because proteolytic enzymes interference will lead to wrong results.
2. Be careful drain reagent and strictly abide by the given incubation time and temperature. Please note that the specimen from the standard /, enzyme combined or substrate, the first hole and the last hole and the time interval between the sample if too big, will lead to different "advance incubation" time, which obviously affect the accuracy of the measurement values and repeatability. And, washing not fully will influence test results.
3. Kit save, part of the reagents store in 20 ℃ -part of the reagents store in 2-8 ℃ , specific to label) on the shall prevail.
4. There will be a strong final twist salting-out, diluted in water heating can help to dissolve.
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