What is YKL-40 ELISA?

YKL-40, aswell alleged animal cartilage glycoprotein-39 (HC gp-39), is a affiliate of ancestors 18 glycosyl hydrolases. YKL-40 is buried by chondrocytes, synovial cells, and macrophages, and afresh it has been appear that YKL-40 has a role as an autoantigen in rheumatoid arthritis (RA). The action of YKL-40 is unknown, but the arrangement of its announcement in accustomed and ache states suggests that it could action in adjustment or abasement of the extracellular matrix. High levels of YKL-40 are activate in synovial aqueous from patients with alive RA. Neutrophils are abounding in synovial aqueous of patients with RA, and the beef are affected to play a role in collective abolition in that disorder. Therefore, we advised whether neutrophils are a antecedent of YKL-40. YKL-40 was activate to colocalize and comobilize with lactoferrin (the a lot of abounding protein of specific granules) but not with gelatinase in subcellular fractionation studies on angry and unstimulated neutrophils. Double-labeling immunoelectron microscopy accepted the colocalization of YKL-40 and lactoferrin in specific granules of neutrophils. Immunohistochemistry on cartilage bottom beef showed that neutrophil precursors activate to amalgamate YKL-40 at the myelocyte-metamyelocyte stage, the date of maturation at which added specific atom proteins are formed. Assuming that YKL-40 has a role as an autoantigen in RA by inducing T cell-mediated autoimmune response, YKL-40 appear from neutrophils in the affronted collective could be capital for this response. In RA and added anarchic diseases, YKL-40 appear from specific granules of neutrophils may be complex in tissue adjustment or degradation.
Chitinase-3-like protein 1 is a protein that in bodies is encoded by the CHI3L1 gene. It is aswell accepted as "YKL-40". Its over-expression has been active in a amount of anarchic diseases and cancer. Additionally, it has been active in asthma, with the analysis of an allele that doubles the risk.
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What is CD11b ELISA Kit?

Specificity    
CD11b ELISA Kit recognizes recombinant and natural rat Integrin alphaM. No significant cross-reactivity or interference was observed.
Principle    
The microtiter bowl provided in this kit has been pre-coated with an antibiotic specific to Integrin alphaM . Standards or samples are again added to the adapted microtiter bowl wells with a biotin-conjugated antibiotic alertness specific for Integrin alphaM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to anniversary microplate able-bodied and incubated. Again a TMB (3,3',5,5' tetramethyl-benzidine) substrate band-aid is added to anniversary well. Only those wells that accommodate Integrin alphaM , biotin-conjugated antibiotic and enzyme-conjugated Avidin will display a change in color. The enzyme-substrate acknowledgment is concluded by the accession of a sulphuric acerbic band-aid and the blush change is abstinent spectrophotometrically at a amicableness of 450 nm ± 2 nm. The absorption of Integrin alphaM in the samples is again bent by comparing the O.D. of the samples to the accepted curve.
Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, aswell accepted as macrophage-1 antigen (Mac-1) or accompaniment receptor 3 (CR3). ITGAM is aswell accepted as CR3A, and array of adverse atom 11B (CD11B). The additional alternation of αMβ2 is the accepted integrin β2 subunit accepted as CD18, and integrin αMβ2 appropriately belongs to the β2 subfamily (or leukocyte) integrins.
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What is U-Calcitonin ELISA Kit?

Calcitonin, a 32-amino-acid polypeptide, is buried primarily by the thyroidal parafollicular C-cells. Its capital biological aftereffect is to arrest osteoclastic cartilage absorption as able-bodied as arena a role in calcium and phosphorus metabolism in the cartilage and kidney. Calcitonin supresses absorption of cartilage by inhibiting the action of osteoclasts absolution calcium and phosphorus into blood. In the kidney, calcium and phosphorus are prevented from getting absent in urine by reabsorption in the branch tubules. Calcitonin inhibits tubular reabsorption of these two ions, arch to added ante of their accident in urine.
Calcitonin is a 32-amino acerbic beeline polypeptide hormone that is produced in bodies primarily by the parafollicular beef (also accepted as C-cells) of the thyroid, and in abounding added animals in the ultimobranchial body. It acts to abate claret calcium (Ca2+), opposing the furnishings of parathyroid hormone (PTH). Calcitonin has been begin in fish, reptiles, birds, and mammals. Its accent in bodies has not been as able-bodied accustomed as its accent in added animals, as its action is usually not cogent in the adjustment of accustomed calcium homeostasis.. It belongs to calcitonin-like protein family.
Calcitonin was extracted from the Ultimobranchial glands (thyroid-like glands) of fish, particularly salmon. Salmon calcitonin resembles human calcitonin, but is more active. At present, it is produced either by recombinant DNA technology or by chemical peptide synthesis. The pharmacological properties of the synthetic and recombinant peptides have been demonstrated to be qualitatively and quantitatively equivalent.
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What is Anti-OVA?

Intended use
Anti-OVA allows for the specific measurement of Guine-pig Anti-OVA-IgE concentrations in cell culture supernates, serum, and plasma.
Introduction
Measurement of anti-OVA-IgE levels after OVA-inoculation to mouse will be helpful in clarification of the immune process.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. OVA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Anti-OVA-IgE present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Anti-OVA-IgE is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-OVA-IgE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Ovalbumin (OVA) is widely used as an antigen for studying allergic diseases in mice. Chondrex provides three types of mouse IgE and one type of IgG anti-OVA antibody assay kit. These IgE assay kits employ an identical IgE standard (Clone E-C1), which allows for easy determination of total IgE and OVA specific IgE ratios in individual samples.
In addition, rat anti-mouse IgE monoclonal antibodies (Clone 345-2 and 77-1) used in these kits react to both IgEa and IgEb allotypes. Therefore, separate assays for IgEa and IgEb allotypes are not required.
This kit is designed to simply determine anti-OVA IgE levels using an OVA-coated ELISA plate for samples used for in vitro experiments, such as an IgE solution used for stimulating mast cells.  This ELISA kit includes reagents to measure 40 samples.  The assay range is approximately 0.8-50 ng/ml.
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What is Phosphatidic Acidc ELISA?

Intended use
Phosphatidic Acidc ELISA allows for the specific measurement of phosphatidic acid concentrations in plant tissue, cell culture supernates and other relevant liquid.
Introduction
Phosphatidic acid (PA) is a common phospholipid that is a major constituent of cell membranes. Phosphatidic acid is the smallest of the phospholipids. Phosphatidic acid is not an abundant lipid constituent of any living organism, but it is extremely important as an intermediate in the biosynthesis of triacylglycerols and phospholipids. Indeed, it is often over-estimated in tissues as it can arise by inadvertent enzymatic hydrolysis during inappropriate storage or extraction conditions during analysis.
Phospholipase D activity and the phosphatidic acid produced may be even more significant in plants. They have long been recognized as of importance during germination and senescence, and they appear to have a role in response to stress damage and pathogen attack. A high content of phosphatidic acid induced by phospholipase D action during wounding or senescence brings about a loss of the membrane bilayer phase, as a consequence of the conical shape of this phospholipid in comparison to the cylindrical shape of structural phospholipids. As a result, cells lose their viability. The phosphatidic acid generated in this way is broken down further by phosphatases, acylhydrolases and lipoxygenases into fatty acids and other small molecules, which are subsequently absorbed and recycled. In addition, phosphatidic acid is important in the response to other forms of stress, including osmotic stress (salinity or drought), cold, and oxidation, although much remains to be learned of the mechanism by which it exerts its effects.
Phosphatidic acid is of considerable importance in cellular signalling in plants, for example in promoting pollen-tube growth, decreasing peroxide-induced cell death, and mediating the signalling processes that lead to responses to the plant hormone abscisic acid. Thus in the ‘model’ plant Arabidopsis, which contains twelve distinct members of the phospholipase D family, phosphatidic acid generated by the action of the enzymes interacts with a protein phosphatase to signal the closure of stomata promoted by abscisic acid; it interacts also with a further enzyme to mediate the inhibition of stomatal opening effected by abscisic acid. Together these reactions constitute a signalling pathway that regulates water loss from plants.
Test principle
Phosphatidic Acidc ELISA employs the quantitative sandwich enzyme immunoassay technique. A antibody specific for phosphatidic acid has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any phosphatidic acid present is bound by the immobilized antibody. An enzyme-linked antibody specific for phosphatidic acid is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of phosphatidic acid bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Where to get Chlamydia Trachomatis IgG ELISA Kit?

Chlamydia Trachomatis IgG ELISA Kit is intended for the qualitative determination of IgG class antibodies against Chlamydia trachomatis in human serum or plasma (citrate). Chlamydia trachomatis IgG ELISA kit is a qualitative immunoenzymatic determination of IgG-class antibodies against Chlamydia trachomatis is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells of the Chlamydia trachomatis IgG ELISA kit are precoated with Chlamydia trachomatis antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured Chlamydia trachomatis -specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product using this Chlamydia trachomatis IgG ELISA kit is proportional to the amount of Chlamydia trachomatis -specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
The reagents of Chlamydia Trachomatis IgG ELISA Kit are stable up to the expiry date stated on the label when stored at 2...8 °C. The ready to use breakapart snap-off strips of this Chlamydia trachomatis IgG ELISA kit are coated with Chlamydia trachomatis antigen. Store at 2...8°C. Immediately after removal of strips, the remaining strips should be resealed in the aluminium foil along with the desiccant supplied and stored at 2...8 °C; stability until expiry date.
The Diagnostic Automation ELISA, Chlamydia Trachomatis IgG is intended for use in evaluating a patient's serologic status to Chlamydia Trachomatis infection. It is also used to evaluate paired sera for the presence of a significant increase in specific IgG as indicative of a recent or current Chlamydia Trachomatis infection.
Purified Chlamydia Trachomatis antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Chlamydia Trachomatis IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.
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What is Vitamin d binding protein ELISA Kit?

Vitamin d binding protein ELISA Kit is a sandwich assay for VDB determination in serum, plasma and urine samples. The wells of the micro titer plate are coated with polyclonal anti-VDB antibodies. In a first incubation step, the VDB in the samples is bound to the coated polyclonal rabbit antibodies (in excess). To remove all unbound substances, a washing step is carried out. In a second incubation step, a polyclonal Peroxidase-labeled rabbit-anti-VDB antibody is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, Tetramethylbenzidine. An acidic stopping solution is then added. The color converts to yellow. The intensity of the yellow color is directly proportional to the VDB concentration in the sample. A dose response curve of the absorbance (at 450 nm) unit vs. concentration is generated.
INTENDED USE
Vitamin d binding protein ELISA Kit Assay is intended for the quantitative determination of free, not Actin-bound, Vitamin-D binding protein in serum, plasma and urine. For in vitro diagnostic use only.
CLINICAL RELEVANCE
Vitamin D-binding protein (VDB; MW = 51 243 Da, positions 17–474, 458 amino acids, P02774 VTDB_HUMAN) or Gc-globulin is a multifunctional serum protein synthesized in the liver. It is structurally related to albumin and is similar in size. The majority of vitamin D in the blood circulates bound to the VDB. Gc-globulin has been reported to be a macrophage-stimulating factor, to possess chemotaxin activity and to have endotoxin-binding capacity.
Furthermore, Gc-globulin has one actin-binding site and forms 1:1 complexes with monomeric actin. Actin is an intracellular protein that can polymerise and form filaments. The mobility and the shape of cells depends on this ability.
Upon massive cell death and tissue destruction, the release of actin may lead to a significant decrease in the components of the extracellular actin scavenger system. Decreased VDB levels were found in serum samples from several patients groups at risk of developing multi-organ failure, e.g. trauma, spesis etc.
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Characteristics of Orphanin FQ kit

Characteristics of Orphanin FQ kit  
For the quantitative determination of human 0-FQ concentrations in serum, plasma,cell culture supernates and tissue homogenate.
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Mouse Orphanin FQ (OFQ) in serum, plasma and other biological fluids.
Orphanin FQ (or Nociceptin ) is the endogenous ligand for the nociceptin receptor.  Nociceptin is thought to be an endogenous antagonist of dopamine transport that may act either directly on dopamine or by inhibiting GABA to affect dopamine levels. Within the central nervous system its action can be either similar or opposite to those of opioids depending on their location.  It controls a wide range of biological functions ranging from nociception to food intake, from memory processes to cardiovascular and renal functions, from spontaneous locomotor activity to gastrointestinal motility, from anxiety to the control of neurotransmitter release at peripheral and central sites.
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What is HSV-1 2 IgM ELISA Kit?

Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There
are two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with
oral infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions
below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with
numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. DIAGNOSTIC AUTOMATION ELISA HSV IgM is an accurate serologic method to
detect HSV specific antibody IgM in serum sample.
Purified HSV antigen is coated on the surface of microwells. Diluted patient serum is added to wells,
and the HSV IgM specific antibody, if present, binds to the antigen. All unbound materials are washed
away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic
reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount
of IgM specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.
HSV-1 2 IgM ELISA Kit is an enzyme linked immunosorbent assay (ELISA) for the diction of IgM class antibodies to HSV-1 in human serum or plasma. HSV-1 and 2 are virtually identical, sharing approximately 50% of their DNA and have over 80% of common antigens. Both types infect the body's mucosal surfaces, usually the mouth or genitals, and then establish latency in the nervous system. For both types, at least two-thirds of infected people have no symptoms, or symptoms too mild to notice. However, both types can recur and spread even when no symptoms are present. By the time they're teenagers or young adults, about 50% of Americans have HSV-1 antibodies in their blood. By the time they are over age 50, some 80-90% of Americans has HSV-1 antibodies. By comparison, almost all HSV-2 is encountered after childhood, when people become sexually active. HSV type 1 is the cause of most orofacial herpes and HSV encephalitis; type 2 is the primary cause of initial and recurrent genital herpes and neonatal HSV. Reactivation of latent HSV infection is a frequent complication of immunosuppression due to cancer, transplantation and AIDS. Asymptomatic genital shedding of HSV-2 is more common than HSV-1 and occurs more frequently during the first 3 months after acquisition of primary type 2 disease than during later periods. The presence of HSV IgG antibody is indicative of previous exposure A significant increases in HSV IgG is an indicative of reactivation, current or recent infection. IgM antibody is present after primary HSV infection.
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What is Melatonin ELISA Kit?

Melatonin ELISA Kit is an enzyme immunoassay for the in-vitro diagnostic quantitative determination of melatonin in human serum and plasma. Melatonin ELISA kit assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the melatonin ELISA kit system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.
The melatonin ELISA kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sunlight. The storage and stability of specimen and prepared reagents is stated in the protocol insert. The microtiter strips of this melatonin ELISA kit are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C. After elution with methanol the Extraction Columns may be used for extraction of the next samples or stored at 2-8°C protected from dust. Extraction Columns may be re-used up to 4 times.
The results obtained using this melatonin ELISA kit themselves should not be the only reason for any therapeutical consequences. They have to be correlated to other clinical observations and diagnostic tests. A study with apparently healthy subjects using this melatonin ELISA kit has shown that the melatonin levels in humans have a marked circadian rhythmicity characterised by very low levels during day-time and high levels during night-time, and show a considerable inter-individual variation. Furthermore, the melatonin concentration is age dependent. The highest concentrations were found in samples of infants (up to 3 years). In a group of six healthy volunteers the circadian rhythm of melatonin was studied. The mean value reaches a minimum of about 4.6 pg/ml during daytime at 4 p. m. and a maximum of about 77.5 pg/ml during night time at 4 a.m. The nocturnal melatonin peak among healthy individuals varies significantly. It is recommended that each laboratory establishes its own range of normal values.
Melatonin has its highest levels in plasma during night time. Its characteristic nocturnal surge appears to encode temporal information such as length of night. Regulation of the melatonin secretion is under neural control. Sympathetic innervation seems to play a major role via its release of noradrenaline. Altered patterns and/or levels of melatonin secretion have been reported to coincide with sleep disorders, “jet lag“, depression, stress, schizophrenia, hypothalamic amenorrhea, pregnancy, anorexia nervosa, some forms of cancer, immunological disorders as well as control of sexual maturation during puberty. Most of the circulating melatonin is metabolized in the liver to 6-hydroxymelatonin and subsequently to 6- sulfatoxymelatonin which is excreted into the urine.
Melatonin ELISA Kit is a Solid phase ELISA based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the we
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What is Anti-Lgr5/GPR49?

Lgr5 (Leucine-rich repeat G protein-coupled receptor 5) is thought of as a possible stem cell marker for the hair follicle and the epithelium of the intestine. LGR5 is only expressed in cycling crypt base columnar cells, which are thought to be genuine intestinal stem cells. LGR5 has also been implicated as a negative regulator of the Wnt signaling pathway. LGR5 is often up-regulated in certain cancers and may play a role in cancer cell progression.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
FUNCTION: Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
Specificity:
This antibody recognizes LRG5 at the cytoplasmic domain.
Format of Anti-Lgr5/GPR49
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Applications of Anti-LYVE-1(lymphalic vessel endotheilial hyaluronan receptor 1)

Immunogen of Anti-LYVE-1(lymphalic vessel endotheilial hyaluronan receptor 1)  
KLH conjugated synthetic peptide derived from mouse LYVE-1 terminus
Format Lyophilized
Reconstitution Reconstitute with distilled water
Isotype IgG
Description    
Synonym: lymphalic vessel endotheilial hyaluronan receptor 1, CRSBP 1, CRSBP1, extracellular link domain containing 1, extracellular link domain-containing 1, HAR, hyaluronic acid receptor, Lymphatic endothelium specific hyaluronan receptor, LYVE 1, LYVE-1, XLKD1.
Applications of Anti-LYVE-1(lymphalic vessel endotheilial hyaluronan receptor 1)   
Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC)
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Characteristics of SCC ELISA Kit

Characteristics of SCC ELISA Kit 
This immunoassay allows for the in vitro quantitative determination of human SC concentrations in serum, plasma and other biological fluids.
Description    
The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
Specificity    
SCC ELISA Kit recognizes recombinant and natural human SC. No significant cross-reactivity or interference was observed.
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How to get Flt3 Ligand ELISA Kit?

Flt3 Ligand ELISA Kit (Enzyme-Linked Immunosorbent Assay) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Flt3 ligand in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Flt3 ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Flt3 ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman Flt3 ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Flt3 Ligand is a ligand for the FLT3 tyrosine kinase receptor and belongs to a small group of growth factors that regulate proliferation of early hematopoietic cells. Multiple isoforms of Flt3 ligand have been identified. The predominant form is the transmembrane form, which is biologically active on the cell surface. When proteolytically cleaved the transmembrane isoform generates a soluble form, which is also biologically active. Flt3 ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3 ligand alone cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation.
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What is Anti-TSGF(Tumor Specific Growth Fanctor)?

Intended Use: TSGF(Tumor Specific Growth Fanctor) ELISA Kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of TSGF in the sample, this TSGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus TSGF concentration. The concentration of TSGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of Anti-TSGF(Tumor Specific Growth Fanctor)
This TSGF enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TSGF. Standards or samples are then added to the microtiter plate wells and TSGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TSGF present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TSGF are added to each well to "sandwich" the TSGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain TSGF and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
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What is Anti-Microsporidia?

Microsporidia constitute a phylum of spore-forming unicellular parasites. They were once thought to be protists but are now known to be fungi. Loosely 1500 of the probably more than one million species are named now. Microsporidia are restricted to animal hosts, and all major groups of animals host microsporidia. Most infect insects, but they are also responsible for common diseases of crustaceans and fish. The distinguished species of microsporidia usually infect one specific host or a related group of hosts. Several species, most of which are opportunistic, also infect humans.
Approximately 10 percent of the Microsporidia are parasites of vertebrates, including in humans.
The microsporidia often cause chronic, debilitating diseases rather than lethal infections. Effects on the host include reduced longevity, fertility, weight, and general vigor. Vertical transmission of microsporidia is frequently reported. In the case of insect hosts, vertical transmission often occurs as transovarial transmission, where the microsporidian parasites pass from the ovaries of the female host into eggs and eventually multiply in the infected larvae. Amblyospora salinaria n. sp. which infects the mosquito Culex salinarius Coquillett, and Amblyospora californica which infects the mosquito Culex tarsalis Coquillett, provide typical examples of transovarial transmission of microsporidia.
Microspridiosis is an opportunistic intestinal infection that causes diarrhea and wasting in immunocompromised individuals (HIV, for example). It results from different species of microsporidia, a group of microbial (unicellular) fungi.
In HIV infected individuals, microsporidiosis generally occurs when CD4+ T cell counts fall below 100.
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How to get Echino ELISA Kit?

Echino indicates spiny or prickly echinoderm coming from New Latin, via Latin from Greek ekhinos sea urchin, hedgehog.
Enzyme-linked immunosorbent assay (ELISA), is a popular format of a "wet-lab" type analytic biochemistry assay that uses one sub-type of heterogeneous, solid-phase enzyme immunoassay (EIA) to detect the presence of a substance in a liquid sample or wet sample.ELISA can perform other forms of ligand binding assays instead of strictly "immuno" assays, though the name carried the original "immuno" because of the common use and history of development of this method. The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection reagent that will bind specifically and use an enzyme to generate a signal that can be properly quantified. In between the washes only the ligand and its specific binding counterparts remain specifically bound or "immunosorbed" by antigen-antibody interactions to the solid phase, while the nonspecific or unbound components are washed away. Unlike other spectrophotometric wet lab assay formats where the same reaction well (e.g. a cuvette) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase which is part of the plate and thus are not easily reusable.
The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.
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Where to find Corticosteroids ELISA Kit?

Description of Corticosteroids ELISA Kit
Synthetic corticosteroids are widely used for veterinary applications. The anti-inflammatory effects of corticosteroids have led to their use in the treatment of pain and inflammation in diseases such as rheumatic fever, rheumatoid arthritis and some acute kidney disease. This use of corticosteroids is legal. Their presence in the body fluids of horses during racing is considered a doping offence. Their use as growth promoters in livestock production has also been reported and is forbidden within the EU (EC directive 86/469). Corticosteroids residues can remain in meat after animals are slaughtered and enter the food chain. The control of these drugs is of the utmost importance owed to their high potency, they are effective in low doses, with results in low residue levels in urine samples. Analysis of compounds in body fluids at low concentration generally requires sample concentration before quantification, resulting in labour-intensive and time-consuming methods. The main feature of corticosteroid ELISA is the simplicity of the method. The only sample preparation required is a 10 fold dilution in buffer i.e. there is no deconjugation procedure required to remove glucuronic acid without hydrolysis of urine samples.
The main feature of Corticosteroids ELISA Kit is the simplicity of the method. The only sample preparation required is a 10 fold dilution in buffer i.e. there is no deconjugation procedure required to remove glucuronic acid without hydrolysis of urine samples.
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What is Anti-Parvalbumin?

Parvalbumin is a calcium binding albumin protein. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is localised in fast-contracting muscles, where its levels are highest, and in the brain and some endocrine tissues. Parvalbumin is present in GABAergic interneurons in the nervous system, predominantly expressed by chandelier and basket cells in the cortex.
Anti-Parvalbumin will react with parvalbumin from brain and muscle. By immunoblot it recognizes a protein of 12 kDa. The antibody is directed against an epitope at the first Ca+2-binding site and specifically stains the Ca+2 -bound form of parvalbumin.
Immunogen
frog muscle parvalbumin.
Specificity
Recognizes Parvalbumin in a Ca2+ ion-dependent manner. Does not react with other members of the EF-hand family such as calmodulin, intestinal calcium-binding protein, S100A2 (S100L), S100A6 (calcyclin), the α chain of S-100 (i.e. in S-100a and S-100ao), or the β chain (i.e. in S-100a and S-100b).
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Advantages of Homocysteine ELISA kit

Test Principle of Homocysteine ELISA kit
The Kit is a solidoid sandwich assay enzyme-linked immunosorbent assay (ELISA). A known concentration of standard, unknown concentration of sample by adding micro-ELISA-plate for testing. First incubated it, and biotin-marked antibodies at the same time. After washing, adding the avidin HRP-marked. After another incubation and washing to remove uncombined enzyme combo, and then adding substrate A, B, and enzyme combo acted simultaneously. Produce color. The depth of color and the concentration of samples was proportional relationship.
The presence of elevated total homocyst(e)ine levels in plasma or serum has been connected to the increased risk of thrombosis, neural tube defects and atherosclerotic vascular disease. With the steadily growing demand for testing, laboratories require fast, reliable determinations that are cost-effective to process. Bio-Rad supplies two kits for homocyst(e)ine analysis: Homocyst(e)ine by HPLC and Homocyst(e)ine by EIA. Bio-Rad's expertise in both HPLC and immunoassay technology assures you of quality results and efficient workflow.
Advantages of Homocysteine ELISA kit
Highly specific enzymatic sample pretreatment
Convenient microplate format
Standardized immunoassay procedure
Parallel sample processing
Wide dynamic range
Excellent correlation with reference HPLC method
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Characteristics of ApoC1 ELISA Kit

Characteristics    
ApoC1 ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of APOC1 in human serum, plasma and other biological fluids.
Alternative name    
Apolipoprotein C1 (APOC1)
Description    
ApoC1 ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of APOC1 in human serum, plasma and other biological fluids.
Specificity    
ApoC1 ELISA Kit has high sensitivity and excellent specificity for detection of human APOC1. No significant cross-reactivity or interference between human APOC1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between human APOC1 and all the analogues, therefore, cross reaction may still exist.
Sensitivity    
The minimum detectable dose of human APOC1 is typically less than 0.69ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero standard added by their three standard deviations.
Function:
Appears to modulate the interaction of APOE with beta-migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification. Ref.13
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How to get Leishmania ELISA?

Leishmania is a astringent ache with top mortality, acquired by bacteria associates of the L. donovani circuitous (1,2). The agent for manual is the beach fly, whose carriers of infection aretypically dogs (3-6). It is a ache ancient to abounding countries and is a austere botheration in abounding developing nations, decidedly with the accretion urbanization of populations (7). Top accident is encountered in locations of Latin America, East Africa, Middle East, India and China. It is ancient to countries adjoining the Mediterranean such as Italy, Southern France, Spain, Portugal, and Northern Africa. In Southern Europe, VL has become the arch adept infection in AIDS patients (8-15). Diagnosis of astute VL is generally attempted by aspiration of cartilage bottom for absolute bacteria identification. The action is invasive, painful, alarming and has a low success amount due to the disability to consistently abstract parasites from the tissue. Alternatively, serodiagnosis is broadly activated back anti-leishmanial antibiotic titers are about top during the astute ache phase. ELISA is the adopted class analysis for
serodiagnosis of Leishmaniasis, although aberrant immunofluorescent antibiotic tests (IFAT) and absolute abutment tests (DAT), application accomplished parasites, are still broadly acclimated in affiliation with ELISA or abandoned (16-18).
During the aboriginal incubation, the antibodies in the patient's serum bind to the antigens in the analysis well. The next evolution allows the antigen-antibody circuitous to bind to an agitator complex. After abrasion the wells to abolish absolved enzyme, a chromogen is added that develops a dejected blush in the attendance of the agitator circuitous and peroxide. The stop band-aid ends the acknowledgment and turns the dejected blush to yellow.
Leishmania is a genus of Trypanosomatid protozoa, and is the parasite responsible for the disease leishmaniasis. It is spread through sandflies of the genus Phlebotomus in the Old World, and of the genus Lutzomyia in the New World. Their primary hosts are vertebrates; Leishmania commonly infects hyraxes, canids, rodents, and humans. Leishmania currently affects 12 million people in 88 countries.
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What is NCAM ELISA kit?

Features of NCAM ELISA kit
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human CD56 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4℃ and eight months at -20℃.
Description
Neural Cell Adhesion Molecule,NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
Principle
The human CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human CD56 specific-specific monoclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD56 amount of sample captured in plate.
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Where to get Endotoxin ELISA Kit?

Reagent Preparation of Endotoxin ELISA Kit 
1. Bring all kit components and samples to room temperature (18-25 ℃) before use.
2. Dispense 10 uL of LYSIS BUFFER SOLUTION into 100 uL specimens, mix and stand for one hour (The proportion of LYSIS BUFFER and Specimens should be no less than 1:10). (NOTE: This step is required when the sample is cell culture fluid & body fluid & tissue homogenate; if the sample is serum or blood plasma, then this step should be skipped.)
3. Wash Solution - Dilute 10 mL of Wash Solution concentrate (100×) with 990 mL of deionized or distilled water to prepare 1000 mL of Wash Solution (1×).
Application ELISA
Storage of Endotoxin ELISA Kit
All kit capacity can be stored at 2-8 degree. From a user angle this is added acceptable if it comes to carriage and accumulator than a claim for freezing some reagents, as accepted for abounding added ELISA kits.
Reliability
Thanks to just one incubation- and abrasion footfall the Elisa agreement can be performed aural two hours. Compared to the acceptable Elisa method, beneath administration accomplish abate errors and bear added constant results. Thorough and approved tests of the arrangement agreement a abundant after and inter appraisal believability and ensure a low accessory of variation.
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Where to find Anti-5-HT(5-hydroxytryptamine)?

Description of Anti-5-HT(5-hydroxytryptamine)
Anti-5-HT(5-hydroxytryptamine) is directed adjoin conjugated anti-Serotonin antibodies. Anti-idiotypic antibodies are developed to mimick the physiological action of biomolecules.
Serotonin or 5-HT(5-hydroxytryptamine) is a monoamine neurotransmitter, biochemically acquired from tryptophan, that is primarily begin in the gastrointestinal (GI) tract, platelets, and axial afraid arrangement (CNS) of bodies and animals. It is a acclaimed contributor to animosity of well-being.
Serotonin is eventually metabolized to 5-HIAA by the liver, and excreted by the kidneys. One blazon of tumor, alleged carcinoid, sometimes secretes ample amounts of serotonin into the blood, which causes assorted forms of the carcinoid affection of flushing, diarrhea, and affection problems. Because of serotonin's advance announcement aftereffect on cardiac myocytes, bodies with serotonin-secreting carcinoid may ache a appropriate affection (tricuspid) valve ache syndrome, acquired by admeasurement of myocytes assimilate the valve.
Applications ELISA, Immunohistochemistry (IHC)
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What is Anti-CYP450(Cytochrome P450 monooxygenase)?

Cytochrome P450 monooxygenase superfamily (officially abbreviated as CYP) is a ample and assorted accumulation of enzymes. The action of a lot of CYP enzymes is to activate the blaze of amoebic substances. The substrates of CYP enzymes cover metabolic intermediates such as lipids and steroidal hormones, as able-bodied as xenobiotic substances such as drugs and added baneful chemicals. CYPs are the above enzymes complex in biologic metabolism and bioactivation, accounting for about 75% of the absolute amount of altered metabolic reactions. 
Cytochrome P450 (CYPs) accord to the superfamily of proteins absolute a heme cofactor and, therefore, are hemoproteins. CYPs use a array of baby and ample molecules as substrates in enzymatic reactions. Often, they anatomy allotment of multi-component electron alteration chains, alleged P450-containing systems. Cytochromes P450 accept been called on the base of their cellular (cyto) area and spectrophotometric characteristics (chrome): if the bargain heme adamant forms an adduct with CO, P450 enzymes blot ablaze at wavelengths abreast 450 nm, identifiable as a appropriate Soret peak.
CYP enzymes accept been articular in all domains of life, i.e., in animals, plants, fungi, protists, bacteria, archaea, and even viruses. More than 11,500 audible CYP proteins are known.
Human CYPs are primarily membrane-associated proteins amid either in the close film of mitochondria or in the endoplasmic cloth of cells. CYPs metabolize bags of autogenous and exogenous chemicals. Some CYPs metabolize alone one (or a actual few) substrates, such as CYP19 (aromatase), while others may metabolize assorted substrates. Both of these characteristics annual for their axial accent in medicine. Cytochrome P450 enzymes are present in a lot of tissues of the body, and play important roles in hormone amalgam and breakdown (including estrogen and testosterone amalgam and metabolism), cholesterol synthesis, and vitamin D metabolism. Cytochrome P450 enzymes aswell action to metabolize potentially baneful compounds, including drugs and articles of autogenous metabolism such as bilirubin, principally in the liver.
The Animal Genome Project has articular 57 animal genes coding for the assorted cytochrome P450 enzymes.
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What is Anti-NGF-β?

Nerve advance factor-beta (NGF-β) is a almighty neurotrophic agency which supports the advance and survivability of assumption and/or glial cells. The alive anatomy of animal beta-NGF is is a noncovalently disulfide-linked homodimer, formed by two 13.5 kDa polypeptide monomers (238 absolute amino acids). The three disulfide bonds are appropriate for biological activity.
Nerve advance factor (NGF) is one of a ancestors of neurotrophins that abet the adaptation and admeasurement of neurons. In corpuscle culture, NGF induces the accumulation of neurite projections and, in vivo, may activate the action of tissues. NGF plays a role in the repair, regeneration, and aegis of neurons, and as such could serve as a ameliorative abettor in neurodegenerative altitude such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of affectionate and acoustic neurons; neurotrophic factor; cutaneous innervation; growth, adverse and adaptation of B lymphocytes. It aswell has a accessible role in abhorrence and tissue repair. NGF is begin in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular bland beef cells, fibroblasts, mast beef and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.
Application Notes:
Immunohistochemistry Analysis: A 1:50-100 concoction from a antecedent lot detected NGFβ in animal academician tissue.
Immunocytochemistry Analysis: A 1:100-250 concoction from a antecedent lot was acclimated in IC.
Usage Statement
Unless contrarily declared in our archive or added aggregation affidavit accompanying the product(s), our articles are advised for analysis use alone and are not to be acclimated for any added purpose, which includes but is not bound to, crooked bartering uses, in vitro analytic uses, ex vivo or in vivo ameliorative uses or any blazon of burning or appliance to bodies or animals.
Applications
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
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Where to find Anti-OPCML (opioid binding protein/cell adhesion molecule-like)?

Immunogen
Recombinant fragment, corresponding to amino acids 75-341 of Human OPCML (BC117254).
Defects in OPCML are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Description
Opioid binding protein/cell adhesion molecule-like(OPCML), (also called opioid-binding cell adhesion molecule (OBCAM)). OPCML is a member of the IgLON family of immunoglobulin (Ig) domain-containing glycosylphosphatidylinositol (GPI)-anchored cell adhesion molecules, as an excellent candidate for the 11q25 ovarian cancer TSG in EOC. The OPCML gene comprises 7 exons, spans approximately 600 kb, and is transcribed from telomere to centromere . And due to the lack of transmembrane domains necessary for signal transduction, it is improbable that OBCAM acts independently as an opioid receptor, more likely, it plays an important accessory role in opioid receptor function.
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Where to get Anti-TLR4 (Toll-like receptor 4)?

Anti-TLR4 (Toll-like receptor 4) as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. 
Toll-like receptor 4(TLRs) are a class of proteins that play a key role in the innate immune system. They are single, membrane-spanning, non-catalytic receptors that recognize structurally conserved molecules derived from microbes. Once these microbes have breached physical barriers such as the skin or intestinal tract mucosa, they are recognized by TLRs, which activate immune cell responses.
TLRs are a type of pattern recognition receptor (PRR) and recognize molecules that are broadly shared by pathogens but distinguishable from host molecules, collectively referred to as pathogen-associated molecular patterns (PAMPs). TLRs together with the Interleukin-1 receptors form a receptor superfamily, known as the "Interleukin-1 Receptor/Toll-Like Receptor Superfamily"; all members of this family have in common a so-called TIR (Toll-IL-1 receptor) domain.
Three subgroups of TIR domains exist. Proteins with subgroup 1 TIR domains are receptors for interleukins that are produced by macrophages, monocytes, and dendritic cells and all have extracellular Immunoglobulin (Ig) domains. Proteins with subgroup 2 TIR domains are classical TLRs, and bind directly or indirectly to molecules of microbial origin. A third subgroup of proteins containing TIR domains consists of adaptor proteins that are exclusively cytosolic and mediate signaling from proteins of subgroups 1 and 2.
TLRs are present in vertebrates, as well as in invertebrates. Molecular building blocks of the TLRs are represented in bacteria and in plants, and plant pattern recognition receptors are well known to be required for host defence against infection. The TLRs thus appear to be one of the most ancient, conserved components of the immune system.
In recent years TLRs were identified also in the mammalian nervous system. Members of the TLR family were detected on glia, neurons and on neural progenitor cells in which they regulate cell-fate decision.
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What is Cryptosporidium ELISA Kit?

Cryptosporidium ELISA Kit an in vitro immunoassay for the qualitative determination of Cryptosporidium antigen in feces. It is a double antibody (sandwich) ELISA using an anti-Cryptosporidium antibody to capture the antigen from the stool supernatant. A second anti-Cryptosporidium antibody is then added which sandwiches the captured antigen. This reaction is visualized by the addition of an antisecond antibody conjugated to peroxidase and the chromogen tetramethylbenzidine (TMB). The resulting blue color development indicates the presence of Cryptosporidium antigens being bound by the anti-Cryptosporidium antibodies.
Cryptosporidium is a coccidian parasite that is recognized as a strong enteric pathogen. This organism causes acute infection, even in people with normal immune systems. Cryptosporidium symptoms range from mild to severe diarrhea with a variety of complications.
The infection in people with comprised immune systems is much more severe and can be life-threatening. Passage of up to 12 liters per day of fluid has been reported.
Cryptosporidium infection can occur through several types of transmission. These include animal to human, water contamination, and person-to-person contact. Person-to-person contact may occur between members of the same household, day care centers, and homosexual men.
Cryptosporidum diagnosis was originally done by direct detection, using microscopic examination of stool samples using stains or fluorescent labeled antibodies as the most common procedures. This method relies on an experienced technician and observation of intact organisms. Historically, the method of microscopic examination has had poor proficiency in examinations. So more methods of diagnosis have been investigated.
Features of Cryptosporidium ELISA Kit
Results in less than 2 hours
Specific for Cryptosporidium oocyst antigen
Easy to perform
Highly standardized
High correlation with IFA-confirmed microscopy
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What is Troponin-T ELISA kit?

Description of Troponin-T ELISA kit  
Troponin is a complex of three regulatory proteins that is integral to muscle contraction in skeletal and cardiac muscle, but not smooth muscle. Troponin is attached to the protein tropomyosin and lies within the groove between actin filaments in muscle tissue. Troponin is a component of thin filaments (along with actin and tropomyosin), and is the protein to which calcium binds to accomplish this regulation. Troponin has three subunits, Troponin C, Troponin I, and Troponin T. Individual subunits serve different functions. Troponin C binds to calcium ions to produce a conformational change in TnI. Troponin T binds to tropomyosin, interlocking them to form a troponin-tropomyosin complex. And in a less complicated light, Troponin T modulates contraction of striated muscle. Troponin I binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place.
Troponin-T ELISA kit is a solid phase-phase sandwich enzyme linked immuno sorbent assay (ELISA). Samples, including standards of known target protein concentrations and unknowns are pipetted into these wells. During the first incubation, the target protein antigen and a biotinylated monoclonal antibody specific for target protein are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove the entire unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of target protein present in the samples.
Characteristics    
Troponin-T ELISA kit allows for the in vitro quantitative determination of mouse Tn-T concentrations in cell culture supernates, serum, plasma and other biological fluids.
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